Cloned (Comment) | Organism |
---|---|
expression of HA-tagged wild-type and mutant enzymes in yeast | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E341D | site-directed mutagenesis, the substitution in the loop region of Mrs2p does not influence conductance of the Mg2+ channel | Saccharomyces cerevisiae |
E341K | site-directed mutagenesis, the substitution in the loop region of Mrs2p abolish conductance of the Mg2+ channel | Saccharomyces cerevisiae |
E342D | site-directed mutagenesis, the substitution in the loop region of Mrs2p does not influence conductance of the Mg2+ channel | Saccharomyces cerevisiae |
E342K | site-directed mutagenesis, the substitution in the loop region of Mrs2p abolish conductance of the Mg2+ channel | Saccharomyces cerevisiae |
additional information | construction of the the isogenic mrs2D deletion strain DBY mrs2-1, and the mrs2D/lpe10D double disruptant DBY747 mrs2-2, lpe10-2, Mrs2p coiled-coil mutations results in elevated Mg21 influx into mitochondria | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cobalt(III)-hexaammine | inhibits Mrs2p channels | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, about, recombinant HA-tagged wild-type and mutant enzymes, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | - |
ADP + phosphate + Mg2+/in | - |
? | |
ATP + H2O + Mg2+/out | Saccharomyces cerevisiae DBY747 | - |
ADP + phosphate + Mg2+/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q02783 | - |
- |
Saccharomyces cerevisiae DBY747 | Q02783 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant HA-tagged wild-type and mutant enzymes in yeast, native enzyme partially by preparation of mitochondria | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Mg2+ transport measurements by patch clamp technique | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Mg2+/out | - |
Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
ATP + H2O + Mg2+/out | Mrs2 protein forms a Mg2+-selective channel of high conductance, which has an open probability of about 60% in the absence of Mg2+ at the matrix site, that decreases to about 20% in its presence, the selective channel controls Mg2+ influx into mitochondria by an intrinsic negative feedback mechanism | Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
ATP + H2O + Mg2+/out | - |
Saccharomyces cerevisiae DBY747 | ADP + phosphate + Mg2+/in | - |
? | |
ATP + H2O + Mg2+/out | Mrs2 protein forms a Mg2+-selective channel of high conductance, which has an open probability of about 60% in the absence of Mg2+ at the matrix site, that decreases to about 20% in its presence, the selective channel controls Mg2+ influx into mitochondria by an intrinsic negative feedback mechanism | Saccharomyces cerevisiae DBY747 | ADP + phosphate + Mg2+/in | - |
? | |
ATP + H2O + Ni2+/out | - |
Saccharomyces cerevisiae | ADP + phosphate + Ni2+/in | - |
? | |
ATP + H2O + Ni2+/out | - |
Saccharomyces cerevisiae DBY747 | ADP + phosphate + Ni2+/in | - |
? | |
additional information | with lower conductance, the Mrs2 channel is also permeable for Ni2+, whereas no permeability has been observed for either Ca2+, Mn2+, or Co2+ | Saccharomyces cerevisiae | ? | - |
? | |
additional information | with lower conductance, the Mrs2 channel is also permeable for Ni2+, whereas no permeability has been observed for either Ca2+, Mn2+, or Co2+ | Saccharomyces cerevisiae DBY747 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, about, recombinant HA-tagged wild-type and mutant enzymes, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the CorA-Mrs2-Alr1 superfamily of Mg21 transporters | Saccharomyces cerevisiae |
Mrs2p | - |
Saccharomyces cerevisiae |